Wenting Tang, Hui Zhang, Li Wang, and Haifeng Qian.Journal of Agricultural and Food Chemistry 2015, 63 Identification and Characterization of Antioxidant Peptides from Enzymatic Hydrolysates of Duck Meat. Lu-Sha Wang, Ji-Chao Huang, Yu-Lian Chen, Ming Huang, and Guang-Hong Zhou.Journal of Agricultural and Food Chemistry 2019, 67 Purification and Characterization of Antioxidant Peptides from Alcalase-Hydrolyzed Soybean (Glycine max L.) Hydrolysate and Their Cytoprotective Effects in Human Intestinal Caco-2 Cells. Qiaozhi Zhang, Xiaohong Tong, Yang Li, Huan Wang, Zhongjiang Wang, Baokun Qi, Xiaonan Sui, Lianzhou Jiang.ACS Sustainable Chemistry & Engineering 2021, 9 Biodegradable Anti-Ultraviolet Film from Modified Gallic Acid Cross-linked Gelatin. Linxin Guo, Taotao Qiang, Yangmin Ma, Longfang Ren, Chao Zhu.Evaluating Peptides of Picrorhiza kurroa and Their Inhibitory Potential against ACE, DPP-IV, and Oxidative Stress. Shweta Thakur, Jyoti Chhimwal, Robin Joshi, Manglesh Kumari, Yogendra Padwad, Rajiv Kumar.
This article is cited by 85 publications. It was concluded that natural peptides extracted and isolated from the Jinhua ham by several chromatographic techniques have antioxidant activities.
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The assessment of fractions indicated that the hydrophobic fractions contributed more to free radical scavenging activities than the hydrophilic peptides. The sequence of the antioxidant peptide was identified as Gly-Lys-Phe-Asn-Val. The fraction with highest DPPH radical scavenging activity was further purified and identified using liquid chromatography tandem matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (HPLC-MALDI-TOF/TOF-MS). The peptides were separated using size exclusion chromatography and reversed-phase HPLC. The peptide extracts exhibited great hydroxyl radical scavenging activity and DPPH radical scavenging activity as well as Fe 2+ chelating ability at the concentration of 1 mg/mL, which suggested the presence of peptides with antioxidant activity. The antioxidant activities of the peptides extracted from Jinhua ham were evaluated on the basis of hydroxyl radical scavenging activity, DPPH radical scavenging activity, and Fe 2+ chelating ability.
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